高效液相色谱法分离/蒸发光散射和紫外检测法测定天麻中天麻甙含量
  • 【摘要】

    A normal-phase high performance chromatographic method with evaporative light-scattering detector(ELSD) and diode array detector(DAD), using n-hexane/methanol/ethyl acetate(volume ratio 6∶3∶2) as the ... 展开>>A normal-phase high performance chromatographic method with evaporative light-scattering detector(ELSD) and diode array detector(DAD), using n-hexane/methanol/ethyl acetate(volume ratio 6∶3∶2) as the mobile phase, was established for the direct determination of active constituents-gastrodin(GA) in the ethanol extracts of Gastrodia elata Blume. An silica gel column Zorbax RX-SIL[46 mm (i.d.)×25 cm, 5 μm] was employed. Regression equations revealed the linear relationships(r=0.998 and 0.999) between the peak areas of GA measured by ELSD and DAD and its concentration. The recoveries of GA measured by ELSD and DAD are 104.22%and 97.65%, respectively. The contents of GA measured by ELSD and DAD are 13.9 mg/g(2.79%RSD) and 12.1 mg/g(2.99%RSD) respectively in Gastrodia elata Blume. The detection limits of ELSD and DAD(S/N=3) are 3 mg/L and 1 mg/L respectively. This method is simple, selective and sensitive. 收起<<

  • 【作者】

    魏泱  丁明玉  李红霞 

  • 【作者单位】

    清华大学化学系

  • 【刊期】

    高等学校化学学报 ISTIC SCI PKU 2001年4期

  • 【关键词】

    高效液相色谱法  蒸发光散射检测法  二极管阵列检测法  天麻  天麻甙