Analyzing the phenotypic and functional complexity of lymphocytes using CyTOF (cytometry by time-of-flight)
  • 【DOI】

    10.1038/cmi.2012.16

  • 【摘要】

    The human immune system is equipped with vast numbers of lymphocytes possessing distinct antigen specificities.The enormous repertoire and heterogeneity of lymphocytes ensures their ability to effecti... 展开>>The human immune system is equipped with vast numbers of lymphocytes possessing distinct antigen specificities.The enormous repertoire and heterogeneity of lymphocytes ensures their ability to effectively fight against external microbial invaders,as well as internal aberrant cells.However,the understanding of the specific phenotypes and functions of lymphocytes is hindered by the lack of tools that can be used to visualize this complexity.Fluorescent dyebased flow cytometry,which can simultaneously analyze 8-10 parameters of a single cell,is commonly used in immunological research,but further expansion of the number of parameters (up to 10-18) is limited by the overlapping excitation and emission spectra of different fluorescent dyes. 收起<<

  • 【作者】

    Guobing Chen Nan-ping Weng 

  • 【作者单位】

    Laboratory of Molecular Biology and Immunology

  • 【刊期】

    中国免疫学杂志(英文版)2012年4期

  • 【关键词】

    淋巴细胞  复杂性  飞行时间  表型  抗原特异性  系统配备  细胞功能  异质性