Determination of hemoglobin adduct of a musk xylene metabolite in trout as biomarker of exposure by gas chromatography mass spectrometry
  • 【DOI】

    10.3321/j.issn:1001-0742.2005.06.023

  • 【摘要】

    Musk xylene(MX) is frequently used as fragrances in formulation of personal care products. Quantification of a bound 4-aminoMX(4-AMX) as cysteine adducts in trout hemoglobin(Hb) was made by gas chroma... 展开>>Musk xylene(MX) is frequently used as fragrances in formulation of personal care products. Quantification of a bound 4-aminoMX(4-AMX) as cysteine adducts in trout hemoglobin(Hb) was made by gas chromatography-ion trap-mass spectrometry(GC/MS). The Hb samples were collected from trout after 24 h exposure to MX at 10 μg/g, and or menhaden oil(control). The formation of cysteine-Hb adduct was observed from nitroso derivative of MX, released by alkaline hydrolysis. The released 4-AMX metabolite was extracted in nhexane. The extract was then reduced by evaporation, and analyzed by GC/MS. When similar agreement of mass spectral features and retention time of 4-AMX were obtained in both standard and sample solutions, the presence of 4-AMX metabolite in the Hb was confirmed.The concentration of 4-AMX was found to be 3.1 x 10-6- 6.9 x 10-6 mg/g in the Hb solution. Quantitation was made based on an internal standard, a calibration plot, and response factor. In the non-hydrolyzed and laboratory blank extracts, the 4-AMX metabolite was not detected. Additionally, coeluting and interfering ions were observed in the biological samples. 收起<<

  • 【作者】

  • 【作者单位】

    Department of Chemistry and Biochemistry/Department of Biochemistry and Molecular Biology

  • 【刊期】

    环境科学学报(英文版) ISTIC SCI 2005年6期

  • 【关键词】

    biomarkers  musk xylene metabolite  Hb adducts  fish exposure