Combined use of two membrane-potential-sensitive dyes for determination of the Galvani potential difference across a biomimetic oil/water interface
  • 【摘要】

    The fluorescence behavior of anionic membranepotential- sensitive dyes, bis-(1,3-dibutylbarbituric acid) trimethine oxonol (DiBAC_4 (3)) and bis- (1, 3 - diethylthiobarbituric acid)trimethine oxonol (... 展开>>The fluorescence behavior of anionic membranepotential- sensitive dyes, bis-(1,3-dibutylbarbituric acid) trimethine oxonol (DiBAC_4 (3)) and bis- (1, 3 - diethylthiobarbituric acid)trimethine oxonol (DiSBAC_2(3)), at a biomimetic 1,2-dichloroethane (DCE)/water (W) interface was studied by the mean of potential-modulated fluorescence (PMF) spectroscopy. The respective dyes gave a well-defined PMF signal due to the adsorption/desorption at the DCE/W interface. It was also found that the potentials where the two dyes gave the PMF signals were different by about 100 mV. We then attempted a combined use of the two dyes for determination of the Galvani potential difference across the DCE/W interface. When 40 μM DiBAC4(3) and 15 μM DiSBAC_2(3) were initially added to the W phase, distinctly different spectra were obtained for different interfacial potentials. The ratio of the PMFsignal intensities at 530 and 575 nm (the fluorescence maximum wavelengths for the respective dyes) showed a clear dependence on the interfacial potential. These results suggested the potential utility of the combined use of two dyes for the determination of membrane potentials in vivo. 收起<<

  • 【作者】

    Tatsuya Yoshimura  Hirohisa Nagatani  Toshiyuki Osakai 

  • 【刊期】

    Analytical and Bioanalytical Chemistry EI SCI 2014年14期

  • 【语种】

    eng