Development of an LC-MS/MS method for aromatase inhibitor screening
  • 【摘要】

    Aromatase (CYP 19A1) is a key steroidogenic enzyme that catalyzes the conversion of androgen to estrogen. In this study, a liquid chromatography-tandemmass spectrometry (LC-MS/MS) method for aromatase... 展开>>Aromatase (CYP 19A1) is a key steroidogenic enzyme that catalyzes the conversion of androgen to estrogen. In this study, a liquid chromatography-tandemmass spectrometry (LC-MS/MS) method for aromatase inhibitor screening was developed and validated. The substrate androstenedione was incubated with human CYP 19A1 supersomes in the presence of NADPH for 30 min, and estrone formation was determined by LC-MS/MS analysis. Cortisone was used as internal standard. The incubation mixture was extracted using a liquid-liquid extraction method with ethyl acetate. Chromatographic separation was achieved using a C_(18) column (3.0×50 mm, 2.7 μm) with a mobile phase consisting of 0.1 % formic acid/acetonitrile adopting gradient elution at a flow rate of 0.4 mL/min. The mass spectrometer was operated in positive electrospray ionization mode. The precursorproduct ion pairs used for multiple reaction monitoring were m/z 287→97 (androstenedione), m/z 271→159 (estrone), and m/z 361→163 (IS, cortisone). The developed method met the required criteria for the validation of bioanalytical methods. The validated method was successfully applied to evaluate aromatase inhibitory activity of plants extracts of Simaroubaceae. 收起<<

  • 【作者】

    Myeong Hyeon Park  In Sook Kim  Mi-Sook Dong  Hye Hyun Yoo 

  • 【刊期】

    Analytical and Bioanalytical Chemistry EI SCI 2014年14期

  • 【语种】

    eng